SIH Medium Size:1kg

SIH Medium Size:1kgSIH medium, developed by Hanh, Friehs and Flaschel (2004), is the next step in the development of synthetic media designed to grow D. discoideum in high cell densities. SIH medium is an improved version of FM medium, as developed by Frank and Kessin (1977). The main difference between SIH and FM is the novel addition of Aspartic acid (1. 1 mM) and increased levels of Lysine (8. 5 mM) and Tryptophane (1. 7 mM). Concentrations of Cysteine, Glutamic

2 at 25°C Suspend 28 gram powdered medium in 1 litre distilled water

1% Store at 2 - 8°C in dark

Suspend 19

the “dropped out” supplement

98 Agar 7 Final pH 7

2 at 25°C Suspend 40 gram powdered medium in 1 litre distilled water

2 K2HPO4 9

For maintenance and propagation of Escherichia coli

Cas Number: 001132-61-2

This formulation supports the growth of most strains that are capable of growing on HL5

CAS 25389-94-0

DNA replication inhibitor causing deoxyribonucleotide depletion